Curcumin is a chemical component extracted from the rhizomes of some plants in the ginger family and araceae family. Turmeric contains about 3% to 6% of it. It is a very rare diketone pigment in the plant kingdom and is a diketone compound. It has antioxidant, Alzheimer's prevention, and anti-inflammatory effects.
1. Antioxidant: Curcumin is a plant polyphenol extracted from turmeric. It is also an important active ingredient in turmeric's pharmacological effects. It has antioxidant, oxygen free radical scavenging, liver and kidney protection effects.
2. Prevent Alzheimer's disease: Curcumin can decompose amyloid protein in the brain and prevent the formation of amyloid protein, which helps prevent Alzheimer's disease.
In food production, curcumin is mainly used for coloring sausage products, canned food, sauce and marinated products, and cannot be eaten directly.
Curcumin is a natural phenolic antioxidant extracted from the rhizomes of ginger plants such as turmeric, zedoary, mustard, curry, and turmeric. The main chain is unsaturated aliphatic and aromatic groups, and it is a diketone compound. Commonly used seasonings and food colorings, non-toxic, chemical formula is C21H20O6.
identify
1. Conventional method
The ethanol solution of color reaction sample A should be pure yellow and have light green fluorescence; if concentrated sulfuric acid is added to this ethanol extract, a deep purple-red color will be produced. Color development reaction B: Treat the aqueous solution or dilute ethanol solution of the sample with hydrochloric acid until it begins to turn slightly orange.
Divide this mixture into two parts and add a small amount of boric acid powder or crystals to one part. The color will turn significantly red compared to the part without boric acid. You can also immerse several pieces of filter paper in the ethanol solution of the pigment, dry it at 100°C, and then moisten it with a dilute solution of boric acid (add a few drops of hydrochloric acid). It should be cherry red after drying.
2. Chromatography
Take 5 mL of the test solution (0.01g sample dissolved in 1mL 95% ethanol) and drop it on the thin layer chromatograph (microcrystalline cellulose, 0.1mm), and place it in a container of 3-methyl-1-butanol/ethanol/water/ In the development tank of ammonia (4:4:2:1) mixed solution, the solvent front is raised by 10 to 15 cm. Observed under ultraviolet light after one day and night: there are three yellow spots with Rf between 0.2 and 0.4; the Rf of other spots are between 0.6 and 0.8; all spots show yellow fluorescence under ultraviolet light. 6.95% ethanol solution has a maximum absorption peak at 425 nm.
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